Showing posts with label pediococcus. Show all posts
Showing posts with label pediococcus. Show all posts

Wednesday, February 20, 2013

Wild Yeast Culture Experiments - Homebrew Horny Tank


In a 2007 NHC Presentation, Vinnie Cilurzo (of Russian River Brewing) suggested homebrewers try keeping a Horny Tank (inoculation tank) for maintaining mixed cultures. Used in many of the sour breweries in Belgium, he thought it may be a good way to keep a mixed culture viable without keeping a starter or brewing on a production schedule. A run-down of the process:
  1. Start a normal batch of sour beer with a mixed culture.
  2. Allow the beer to ferment in a plastic bucket for at least three months.
  3. Any time after three months, brew another batch of wort.
  4. Transfer the first batch to secondary for further conditioning, leaving behind a bit of liquid, the yeast slurry, and trub.
  5. Immediately add fresh wort to the fermentor with the wild yeast slurry.
  6. Repeat.
I started my own "Homebrew Horny Tank" last October. I brewed a blonde wort with a traditional turbid mash and inoculated the batch with several doses of revitalized, commercial bottle dregs. I plan to mature the beer in the bucket, tasting every few months.

The fear in using plastic buckets to condition a wild-fermented beer is that the bucket allows too much oxygen diffusion, resulting in excessive acetic acid production. After doing some research, it seems this fear is perpetuated by a single source. Although this article is an incredible resource for brewing sour beer, I believe the plastic bucket deserves a second chance.

To be safe, I will only disturb the beer to take samples, and I will purge the headspace with CO2 after sampling. When the beer is "ready", or if it displays the slightest amount of acetic acid, I will transfer it into a keg, leaving behind about a quart of beer, trub, and slurry. I'll then immediately transfer another batch of wort into the bucket and start the experiment over.


So far the results have been positive. Surprisingly, the bugs from the bottle dregs made quick work of the wort, completing primary fermentation in nine days and forming a pellicle in about two weeks. My first (and most recent) sample was three months after brewday (1/13). As expected, the beer tasted very young, with only a slight prickle of acidity. The aroma had a heavy sulfur note, which I believe will mellow with time (I've had sulfur aromas in a few other young sour brews). More importantly, the beer showed no signs of acetic acid production in the aroma or flavor. So far, so good.

In keeping with the theme of this blog, I want to find mixed culture methods that will translate well to a commercial scale. My mixed culture starter method could be utilized in a commercial brewery, but I believe it will often not be financially or logistically feasible. The brewery may not have the space or funds to allocate a fermentor or yeast propagation vessel specifically for wild yeast. At the same time, banking a special blend, or buying several strains of yeast and bacteria to produce a relatively small quantity of beer can also be extremely expensive.

In addition to those producing only sour and funky beers, I believe the horny tank technique could also work for commercial breweries producing them in limited amounts. Though still taking up fermentor space, it allows the brewer to consistently produce sour beer from the culture, rather than just store the slurry. The horny tank is flexible, allowing the brewery to keep a batch of fermenting sour beer and a viable culture in the same vessel. Though some may call this method sacrilege or assume its a shortcut, the time, resources, and money required to create a barrel-aged sour beer often makes them prohibitive. As homebrewers are creating fantastic funky and sour brews in glass, plastic, and stainless, I believe commercial brewers can do the same.

I will continue to make updates on this experiment as well as others in this series. To be honest - I don't see the series ending. I hope to continue playing with sour beermaking techniques, learning new techniques, finding new bugs, and creating new flavors. Let's face it - you can never have enough good sour beer around the house!

Wednesday, January 30, 2013

Wild Yeast Culture Experiments - Intro and Mixed Culture Method

Brewing (and drinking) sour, funky, "wild" beers is a passion of mine. I always have at least a few microbe-laden brews fermenting. I focus much of my reading and researching on topics surrounding sour beer. For me, brewing with brett and bacteria is a perfect combination of science and alchemy. I am basically the bugs' assistant brewer; preparing the equipment and materials so they can make the magic.

My most successful sour projects have all been fermented with a cocktail of flora, harvested from dregs of my favorite commercial sour beers. Maintaining and fermenting with this mixed culture has yielded significantly more depth and complexity than commercial  yeast/bacteria blends.

A double batch of Flanders Red, split and fermented with Wyeast Roselare (left) and my mixed wild culture (right). The mixed culture portion showed significantly higher levels of acidity and brett character.

I do not have the resources to plate and bank yeast, so I am limited to a macro-scale mixed culture. In the past, it has been as simple as a 1-gallon growler with 2-3 quarts of low gravity wort. The initial pitch is normally either a pack of commercial yeast blend or dregs from a few bottles of young, lower-gravity commercial sours. Prior to pitching the dregs into the starter, I will conduct a small step up with a few ounces of wort in the bottle. This gives the dregs a fighting chance in the mixed culture, and it is a decent test for both contamination and yeast health. The main culture is routinely decanted and fed fresh wort every 3 months or so.

My method is a primitive one, giving up even more control of the finished product to the yeast. However, I have been able to use the culture over a period of 2-3 years, all the while creating beers with strikingly similar  flavor profiles and levels of acidity and attenuation. The most significant differences stem from the additional depth contributed from introducing new species into the culture over time. I have yet to find the "expiration date" of a mixed culture; I have used all of the slurry before noticing any undesirable changes.

This series of posts aims to compare different methods of maintaining wild yeast cultures on a 'macro' level. More to come!

Thursday, January 24, 2013

Wild Yeast Culture Experiments - Separating the Bugs




Over the past few months (or in one case, years), I have gathered commercial cultures of several yeasts and bacteria. The goal for this batch of microbes is to grow and maintain the individual cultures separately. I will use a fair amount of each culture in this year's lambic-esque brew, but I will also keep the individual cultures going for other experiments (berliner weisse and a brett-spiked saison, among others).

I love to collect sour beer dregs from commercial breweries, so I added a bit of the starter wort to a few freshly-consumed bottles. If these bottle cultures are successful, they will start off a fresh mixed culture.

Somewhat of a primer of what's to come, the cultures in this post are part of a set of experiments I started over a year ago. I am currently experimenting with several methods of maintaining mixed wild cultures, and hope to post results in the coming months!


Starter "Brewday": 01/08/13

Per Yeast text: 1g DME for every 10 mL H20

Made 8.5L starter with 850g of Briess Light Dry Malt Extract. Added 1/4 tsp Wyeast yeast nutrient.

Cooled to 80F. Poured into sanitized growlers and left on front porch until 65F.

Pitched following yeast (containers described, just in case labels fall off):


3.5L (1-gallon, clear growler) - 1056 starter
Note: this yeast is just for my next brewday

2L (Sun King growler) - Lacto starter

1L (traveling growler) - Brett Clausenii starter

1L (Flat12 growler) - Pedio starter

Note: As you can see from the picture, I got this Pedio starter for a bargain! I bought it last year, already out of date. The manufacture date on the package is March 2011 (Wyeast recommends using within 6 months), so I am not sure if this culture will yield any viable bugs.

1L (3WM growler) - Sherry Flor starter

~ 0.5L (from over-filled growler) - Split between two 750mL bottles